MCDB101L

Current molecular genetics techniques used to study model organisms. Module1 - Forward genetics: Mutagenesis and genetic mapping of mutants using single nucleotide polymorphisms, classical Mendelian inheritance. 2) Reverse genetics: gene knockdown by RNAi. 3) Genetic analysis of cell signaling. 4) Cellular reprogramming using transgenic strains. Techniques: PCR, gel electrophoresis, Computer based sequence analysis, fluorescence and light microscopy and imaging. Experiments are based on ongoing research at UCSB. Students get to design their own experiment in final module.